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Nitrite stage:
15. Remove all the media and empty the vessel completely and rinse it out. Refill it with brand new dechlorinated water and put the media back in. Then repeat this procedure as the media will still be holding a small amount of water which will be sky high in nitrite. Test the water for nitrite to satisfy yourself you have got rid of most of it.
16. Simply feed the original small amount of ammonia (10 ml) to the new water to give you a controlled nitrite read. Now you will repeat the whole maturing process but this time you will not test for ammonia - but nitrite instead. Once the nitrite has dropped, simply add more ammonia in exactly the same way as before. WATCH that pH! (KH)!
17. You should be able to get the nitrite converting to a similar rate at which you got your ammonia reducing in the first stage. How long this takes depends on how good a 'seeding' you provided in the very first place. Do not worry about the ammonia bugs not getting enough to eat - this doesn't appear to matter. Even if they die back a bit, the speed they come back in the vessel makes this nothing to worry about. Try not to let the nitrite run out, but don't overload it.
Once it is consuming both the ammonia and now nitrite overnight in decent quantities - it is all ready to go into your pond filter. Once it is in, make sure you feed the koi plenty to keep all your hard work alive.
Remember to watch that pH and or KH, as I guarantee it will catch you out at least once, it did me. Occasionally I found the water got totally messed up - i.e. the pH would, for some reason, rocket to way over 9, when this happened it seemed to halt the proceedings. The best way I found around this was to swap the water out again.
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